Fig. 3

YWHAZ is a direct downstream target of miR-185-5p in NCI-H1299 and PC9 cells. A StarBase2.0 was used to predict the potential binding sequences between YWHAZ and miR-185-5p. B Dual-luciferase reporter assay was performed to verify the binding relationship between miR-185-5p and YWHAZ in NCI-H1299 and PC9 cells. C and D The mRNA level of YWHAZ was measured in NCI-H1299 and PC9 cells by qRT-PCR after miR-185-5p mimic (C) or inhibitor (D) transfection. E Western blot was performed to measure the protein level of YWHAZ in NCI-H1299 and PC9 cells transfected with miR-185-5p mimic. F The mRNA expression level of YWHAZ in NSCLC tissues. G The correlation analysis between YWHAZ and miR-185-5p expression level. Each experiments included three biological replicates. The data was displayed as Mean ± SD. **p < 0.01, ***p < 0.001