Fig. 1

STA alleviates H/R‑induced cytotoxicity and oxidative stress in cardiomyocytes. (a) H9c2 cells were subjected to 4 h hypoxia and reoxygenation for 2, 4, 8, 12, or 16 h. (b) H9c2 cells were incubated with STA (0, 10, 20, 50, 100, 200 µM) for 24 h. (c) H9c2 cells were pretreated with 50 µM STA for 2 h, followed by 4 h hypoxia, and 12 h reoxygenation. Cell viability was evaluated by CCK-8 assay. (d) An LDH release assay evaluated cardiomyocyte damage. The colorimetric method determined the Lipid oxidation product MDA (e) and a ROS-scavenging enzyme SOD (f). Values are expressed as Mean ± SD from at least three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001 vs. control group; #P < 0.05, ##P < 0.01, ###P < 0.001 vs. H/R group. STA, Stachydrine; H/R, hypoxia/reoxygenation; LDH, lactate dehydrogenase