Fig. 2

STA inhibits apoptosis in cardiomyocytes with H/R. H9c2 cells were pretreated with 50 µM STA for 2 h and then subjected to 16 h H/R. (a) Apoptotic cells were stained with TUNEL. Representative photomicrographs of H9c2 cardiomyocytes are shown. Magnification, ×50. (b) Quantification of TUNEL staining results. Apoptosis cells were counted from five random fields and normalized to total cells. (c) The caspase-3 activity was measured. Bar: 50 µM. Values are expressed as Mean ± SD from at least three independent experiments. ***P < 0.001 vs. control group; ###P < 0.001 vs. H/R group