Fig. 4

NLRP3 overexpression abolished the suppression of the activated Nrf2/HO-1 pathway on COPD cell pyroptosis. A Western blot revealed the successfully pCDNA3.1-NLRP3 vector-transfected NHBE cells. B Western blot indicated that the pCDNA3.1-NLRP3 vector-transfection reversed the suppression of the activated Nrf2/HO-1 pathway on NLRP3 expression in COPD cells. C Western blot implied that NLRP3 overexpression abrogated the suppression of activated Nrf2/HO-1 pathway on the expression of pyroptosis-related proteins (Caspase-1 and GSDMD-N) in COPD cells. ***P < 0.001 vs. Control group. # P < 0.05 and ## P < 0.01 vs. CSE group. ^^ P < 0.01 and ^^^ P < 0.01 vs. CSE + DMF group