Fig. 2

METTL14-mediated m6A modification promotes the transition of pri-miR-146a-5p into miR-146a-5p. A: m6A levels in cells were determined by a m6A quantification assay kit; B-C: pri-miR-146a-5p and miR-146a-5p expression levels in cells were determined by RT-qPCR; D-E: The binding of DGCR8 and m6A to pri-miR-146a-5p was analyzed by the RIP assay. Each experiment was repeated three times independently. Data were shown as mean ± standard deviation. Data in panels A-C were analyzed by one-way ANOVA and data in panels D-E were analyzed by two-way ANOVA, followed by Tukey’s multiple comparison test. * P < 0.05, ** P < 0.01