Fig. 5

APPL1 downregulation neutralizes the inhibitory role of METTL14 downregulation in the ferroptosis of H/R-induced cardiomyocytes. HL-1 cells were transfected with si-APPL1, with si-NC as the negative control. A: mRNA levels of APPL1 in cells were determined by RT-qPCR; B: Protein levels of APPL1, ACSL4, and GPX4 in cells were determined by Western blot assay; C: Cell viability was evaluated by the CCK-8 assay; D-F: Fe²⁺ content (D), ROS levels (E), and GSH levels (F) were determined by assay kits. Each experiment was repeated three times independently. Data were shown as mean ± standard deviation. Data in panels A and C-F were analyzed by one-way ANOVA, and data in panel B were analyzed by two-way ANOVA, followed by Tukey’s multiple comparison test. * P < 0.05, ** P < 0.01