The incidence of POMC in non-thymoma MG in our study was 11.63%, which is lower than previous studies [5,6,7,8]. Osserman type IIb and above are independent risk factors for our POMC (P < 0.001), which is also a relatively well recognized result [5, 9,10,11,12,13]. These patients have muscle weakness in swallowing, chewing, articulation, and breathing because of the involvement of the bulbar-related muscle groups. In addition to inspiratory and expiratory muscles, facial, oropharyngeal, and laryngeal muscles are important to maintain respiratory function. Weakness in these muscles interferes with upper airway protection, swallowing, and secretion clearance, even lead to upper airway obstruction and respiratory failure . In addition to the above reasons, clinically, we conclude that most of these symptoms are caused by intraoperative tracheal intubation stimulation, pyridostigmine bromide and other reasons that lead to more airway secretions and inability to cough up, plus the saliva is unable to swallow or spit out, which is easy to cause aspiration or blockage of airway. POMC can often not be avoided, even we adjust the dosage of pyridostigmine bromide and anisodamine in real time according to the patient's condition. In order to reduce the airway irritation caused by intraoperative tracheal intubation, almost all the surgical methods have been changed to thoracoscopic thymectomy through the subxiphoid approach in the past two years, enabling us to perform laryngeal mask-assisted ventilation instead of tracheal intubation. However, at present, the amount of data is not enough, and there are many other influencing factors, and the protective advantage to POMC has not yet been shown.
Our multivariate analysis showed that a history of MC was a predictive factors for POMC(P = 0.013), whereas preoperative MG severity was not, which was also mentioned in some other studies . About 20% of MG patients develop MC in their lifetime and one-third of them have a second MC . This means that if the patient has ever had severe MG symptoms, although the symptoms are well controlled before surgery, MC may still occur after surgery, anesthesia and other blows. Therefore, even if the symptoms of these patients are well controlled, they should not be taken lightly. Postoperative management needs to be strengthened to be alert to the occurrence of POMC.
Another independent risk factor for POMC was the preoperative dose of 240 mg or more of pyridostigmine bromide daily (P < 0.001). Other studies  may not be exactly 240 mg, but in general, POMC is more likely to occur at higher dose. Acetylcholinesterase inhibitor (AChEI) increases the concentration of acetylcholine by blocking acetylcholinesterase at the neuromuscular junction to improve muscle strength. The large doses of cholinesterase inhibitors will accelerate the destruction of postsynaptic acetylcholine receptors (AChRs) at the neuromuscular junction, which decreases the postoperative ability of patients to cough up sputum, causes excessive respiratory secretions, increase the risk of respiratory system infection. The above factors will increase the risk of POMC. On the other hand, the larger dosage reflects severer symptoms. Therefore, the dosage of pyridostigmine bromide should be adjusted to the minimum dose range that can control symptoms before surgery as far as possible.
In the past, it seems that no one regarded preoperative comorbidities and basic health status of patients as one of the evaluation indicators of POMC. Clinically, we found that patients with poor preoperative general condition may be more prone to POMC. Therefore, we used the American Society of Anesthesiologists (ASA) (2020 edition) system to assess the preoperative comorbidities and underlying health status of patients. ASA classification is designed primarily to predict and evaluate the risk of anesthesia in surgery, which was first proposed in 1941 . It has been verified by a large number of clinical cases and modified for many times. The ASA score system is also significantly correlated with the incidence of postoperative complications in different surgical specialties [17,18,19,20]. In our study, we found that ASA score was an independent influencing factor of POMC (P = 0.001), indicating that patients with more preoperative comorbidities were more likely to develop POMC. ASA as a predictor of POMC has never been mentioned in previous studies and needs to be further validated.
In our study, 35 patients with Osserman classification IIb or above, and unsatisfactory MG symptom control, received preoperative gamma globulin. As a high-risk group, 13 of them achieved POMC, with an incidence rate of 37.14%. Many studies  believe that gamma globulin can reduce the incidence of POMC. In our univariate analysis, preoperative gamma globulin was a possible risk factor, but in multivariate analysis, it did not become an independent risk factor. However, Gamez et al. conducted a prospective randomized controlled study in 2019 , suggesting that the application of gamma globulin could not reduce the risk of postoperative POMC in patients with well-controlled preoperative MG symptoms.
It must be acknowledged that there are some deficiencies in this study: 1. This is a retrospective study; 2. Limited to conditions, many patients did not have MG-related antibody tests, and many patients only had AChR-Ab, but no MuSK-Ab and other tests; 3. Preoperative treatment is inconsistent, and some of the high-risk patients with POMC were treated with gamma globulin, which inevitably affected the results; 4. Some patients are unable to cooperate with the pulmonary function test before surgery, so the result of the pulmonary function may not be accurate.
Through the study of perioperative factors in 387 non-thymoma MG patients, we concluded that the independent risk factors for POMC were: history of previous crisis, preoperative MG Osserman stage IIb and or above, preoperative dose of pyridoxine bromide ≥ 240 mg, and poor ASA classification. Therefore, non-thymoma MG patients should be highly vigilant if combined with the above conditions, take countermeasures in advance, and timely deal with the MG crisis that may occur at any time to ensure the safety of patients.