- Research article
- Open Access
- Open Peer Review
Initial experience with a synthetic sealant PleuraSeal™ after pulmonary resections: a prospective study with retrospective case matched controls
- Sebastian Dango1Email author,
- Rong Lin2,
- Ellen Hennings1 and
- Bernward Passlick1
https://doi.org/10.1186/1749-8090-5-50
© Dango et al; licensee BioMed Central Ltd. 2010
- Received: 12 March 2010
- Accepted: 16 June 2010
- Published: 16 June 2010
Abstract
The objective of this study was to evaluate postoperative outcome and efficacy of a hydrogel tissue sealant for prevention of alveolar leakage after open lung resections.
20 consecutive patients were enrolled in the PleuraSeal™ sealant group (PSG) and case matched with 20 retrospective controls (CG) with standard treatment. Assessment of postoperative air leakage was performed until chest tube removal. Patients were followed until 30 days after discharge.
At end of surgery, 100% in the PSG and 0% in the CG were air leak free (p < 0.001). Duration of postoperative chest tube suction was shorter in PSG (p < 0.001), and air leak chest tube was removed earlier (p = 0.03). Limitation for chest tube removal due to a pulmonary leak was 35% in CG and 5% in PSG (p = 0.04). Patients remaining air leak free thru discharge was 95% and 15% for PSG and CG (p < 0.001).
The study demonstrated a superior efficacy of PleuraSeal™ sealant compared with standard surgical treatment for sustained sealing of postoperative air leakage and causes shorter air leak chest tube duration.
Keywords
- Chest Tube
- Pulmonary Resection
- Pleural Empyema
- Sealant Group
- Sealant Application
Introduction
Postoperative air leakage remains the most common pulmonary complication in patients undergoing pulmonary resection with a reported occurrence of 18-58% of the cases [1, 2]. Persistent postoperative air leakage (>7 days) has been reported in up to 25% of patients undergoing pulmonary resection [3]. Described risk factors for pulmonary leakage are incomplete fissures [4], underlying lung diseases such as emphysema, fibrosis, tuberculosis or malignancies [5], presence of a lymphangioleiomyomatosis [5], intrathoracic adhesions [6], older patients (>75 years) [7], and lower diffusion capacity [8]. Deleterious effects of prolonged air leaks are longer chest tube duration often associated with prolonged pain [6, 9], prolonged hospitalization [6, 8, 10] and greater health care costs [9, 10], increased risk of pneumonia and empyema [8, 9], decreased postoperative mobility [11], and can necessitate pleurodesis or re-operation [11]. In addition, the incidence of postoperative empyema increases dramatically when air leak is present. As shown by Brunelli et al [12], postoperative empyema was found in 1% and 10% of patients with pulmonary leaks less than and greater than 7 days, respectively.
Surgical suturing and stapling are the standard methods for the prevention and treatment of air leakage and can be challenging especially in patients with fragile lung tissue or emphysema as seen in smokers. Over the last decade, various surgical sealants have been introduced for further prevention and reduction of air leaks. These include liquid autologous fibrin-based sealants [13, 14], collagen fleece-bound sealants [15, 16], and polyethylene glycol (PEG) hydrogel sealants such as PleuraSeal™ [17, 18]. PleuraSeal™ lung sealant is a new, easy to prepare, 100% synthetic, flexible resorbable blue gel that expands with lung inflation and has a sealing strength five-times greater than normal liquid fibrin glue [19]. PleuraSeal™ sealant begins as two liquids that crosslink when mixed; rapidly changing within seconds into a solid hydrogel that mechanically bonds to the underlying tissue. This hydrogel remains in place while natural healing occurs underneath the gel, hydrolyzing like absorbable sutures within 4-8 weeks. The objectives of this study were to evaluate the postoperative outcome, safety, and efficacy of this new hydrogel tissue sealant for the sealing of alveolar leakage after lung resections.
Patients and Methods
Setting and samples
To investigate the efficacy and postoperative outcome, PleuraSeal™ lung sealant system was prospectively evaluated as an adjunct to standard closure techniques for control of visceral pleural air leaks in patients scheduled for pulmonary resection. These data were compared with an individually case matched retrospective control cohort. Sample size calculation was based on a superiority test for two proportions using Fisher's Exact Test, estimated proportion was 72% for PSG and 15% for CG, respectively. Therefore, for an estimated power of 90% a sample size of 18 patients for each group was necessary to show a difference in the two-sided test for the primary endpoints to reach statistical significance. Data were collected at the University Hospital Freiburg, Department of Thoracic Surgery, from patient admission to discharge. Twenty consecutive patients with anatomical resection were included. Inclusion criteria for both groups were patient age 18 years or older, and a scheduled or completed (in retrospective control group) lobectomy or segmental wedge resection via an open thoracotomy approach and presence of an initial intraoperative air leak after resection. Preoperatively, groups were well balanced regarding concomitant pulmonary disease, mean FEV1, lung tissue quality, presence of diabetes and smoking habit. The study was approved by the local ethical committee (340/08, 12/02/08) and written informed consent of all patients was obtained. Only patients with intraoperative air leaks grade 1-2 based on the Macchiarini scale [20] after standard surgical suture or stapling were enrolled. All subjects had two chest tubes during the first 48 hours after thoracotomy. Routinely two silicone chest tubes (ventral 21 Ch and dorsal 24 Ch, silicone chest tubes, Redax Company, Mirandola, Italy) were placed after thoracotomy and connected to a water sealed drainage system. Suction of -20 cm H2O was taken off once no air leaks were detected after 12 hours of observation time. The ventral chest tube (air leak chest tube) was taken out when there was no evidence of an air leak within the last 24 hours, and the dorsal tube was taken out when less than 200 ml drainage was recorded within last 24 hours. Assessment of postoperative air leakage was performed twice daily until chest tube removal and discharge. Patients were discharged and re-evaluated within 30 days after leaving the hospital, with two fixed scheduled visits to an ambulatory outpatient clinic. Primary criteria for discharge were no significant pain (VAS ≤ 2) under present pain medication and absence of chest tube.
Application and technique of PleuraSeal™ Lung Sealant System
Prior to the application of PleuraSeal™ lung sealant an intraoperative air leak test during lung inflation was carried out to evaluate the location and grade of air leaks as described above. In the event of a grade 3 air leak additional suture or stapling was performed to downgrade the pulmonary leak, so that PleuraSeal sealant was applied only in patients with grade 1 or 2 leaks. PleuraSeal™ polymer kit with a total volume of 5 ml was applied using a MicroMyst™ Applicator with a continuous flow (flow regulator) under aseptic conditions to seal intraoperative air leaks as well as prophylacticly on staple lines and other manipulated lung tissue. The lung surface was as dry as possible and the lung was inflated between 50-75% of its maximal volume. The product was uniformly distributed in a layer of 1-2 mm thickness, and on areas of extending pulmonary lesions a thicker layer was achieved of up to 3 mm. Excessive material was mechanically removed using suction or blunt dissection. Contraindications to apply PleuraSeal™ lung sealant include uncontrolled transected bronchioles >1 mm, and intrathorax infections. Additionally, the sealant was not applied to bronchial stumps or bronchial anastomoses. Following sealant application leak sites underwent another water submersion test under pressure of 25 mmHg. Sealant application could be repeated if air leakage control was not halted after the first application.
Clinicopathological Parameters
Baseline characteristics and pre-operative variables
Variablea | Overall | PleuraSeal™ Group | Control Group | p-valueb |
|---|---|---|---|---|
40 | 20 | 20 | ||
Age (yrs.), mean (SD) | 69 (45-82) | 66 (47-81) | 0.34 | |
Gender | ||||
Male | 31 (77%) | 14 (70%) | 17 (85%) | |
Female | 9 (22%) | 6 (30%) | 3 (15%) | 0.45 |
Chronic lung disease | 22 | 11 | 11 | |
COPD | 19 (47%) | 9 (45%) | 10 (50%) | |
Lung emphysema | 2 (5%) | 2 (10%) | 0 (0%) | |
Asthma | 1 (2%) | 0 (0%) | 1 (5%) | 0.58 |
Pulmonary function c | ||||
FEV1 actual (Ø %), mean (SD) | 85.5 (17.9) | 87 (20.2) | 84 (15.6) | 0.51 |
FEV1 predicted (Ø %), mean (SD) | 65.5 (16.2) | 69 (17.6) | 62 (14.8) | 0.18 |
DLCO actual (Ø %), mean (SD) | 70.5 (14.5) | 70 (15.7) | 71 (13.3) | 0.84 |
DLCO predicted (Ø %), mean (SD) | 55 (14.6) | 56 (16.3) | 54 (12.9) | 0.55 |
Neoadjuvant therapy | 1 | 0 | 1 | |
Radiation | 0 (0%) | - | - | NA |
Chemotherapy | 1 (2%) | 0 (0%) | 1 (5%) | 1.00 |
Concomitant disease | 23 | 14 | 9 | |
Diabetes | 7 (17%) | 4 (20%) | 3 (15%) | 1.00 |
Othersd | 16 (40%) | 10 (50%) | 6 (30%) | 0.23 |
Smoking behaviour | ||||
Current Smoker | 15 (37%) | 8 (40%) | 7 (35%) | |
Ex smoker (≥12 month) | 13 (32%) | 6 (30%) | 7 (35%) | |
Never smoked | 12 (30%) | 6 (30%) | 6 (30%) | 0.14 |
Surgical variables and intra-operative findings
Variablea | Overall | PleuraSeal™ Group | Control Group | p-valueb |
|---|---|---|---|---|
40 | 20 | 20 | ||
Surgical diagnosis | ||||
Lung cancer | 38 (95%) | 18 (90%) | 20 (100%) | |
Lung metastasis | 2 (5%) | 2 (10%) | 0 (0%) | 0.48 |
Operation time (hh:mm) | 02:35 | 03:07 | 0.07 | |
Lung tissue quality | 20 | 20 | ||
Normal | 21 (52%) | 10 (50%) | 11 (55%) | |
Fragile | 19 (47.5%) | 10 (50%) | 9 (45%) | 1.00 |
Procedure | ||||
Right lobectomy | 19 (47.5%) | 7 (35%) | 12 (60%) | |
Upper | 13 (32%) | 6 (30%) | 7 (35%) | |
Middle | 0 (0%) | 0 (0%) | 0 (0%) | |
Lower | 6 (15%) | 1 (5%) | 5 (25%) | |
Left lobectomy | 18 (45%) | 11 (55%) | 7 (35%) | |
Upper | 10 (25%) | 7 (35%) | 3 (15%) | |
Lower | 8 (20%) | 4 (20%) | 4 (20%) | |
Segmentectomy | 2 (5%) | 1 (5%) | 1 (5%) | |
Bilobectomy | 1 (2.5%) | 1 (5%) | 0 (0%) | 0.26 |
Intraoperative air leaks characteristics | ||||
Intraoperative leak free (in %) | 100% | 0% | <0.001 | |
Postoperative leak free (in %)c | 95% | 15% | <0.001 | |
Initial # of intraoperative air leaks/patient (Ø)d | 1.6 | 1.6 | 1.6 | 1.00 |
Initial grade of air leaks/patient | 1.35 | 1.3 | 1.4 | 0.77 |
Additional procedure for air leak control | 8 | 2 | 6 | |
Suture | 8 (20%) | 2 (10%) | 6 (30%) | 0.23 |
Staple | 0 (0%) | - | - | NA |
Efficacy and safety endpoints
Three primary endpoints were defined by protocol: reduction of intraoperative air leakage, the presence and duration of postoperative air leak, and postoperative morbidity. Secondary parameters were duration of chest tube placement as well as time to discharge. Adverse events (AE) for the prospectively collected study cohort from the screening period prior to admission to our surgical ward until follow-up of 30-days after discharge were documented and reported if eligible. Complications were defined as occurrence of empyemas, incomplete lung inflation, pneumothorax, presence of bronchogenic fistula, chylothorax, cardiovascular complications, any other organ specific failure, severe postoperative pain, acute esophagitis, and the need for additional procedures (re-operation, pleurodesis, and replacement of chest tube).
Statistical analyses
All statistical analyses were performed using SAS® Version 9.1. All statistical tests were two-sided at the 5% significance level. Continuous variables were summarized using mean and standard deviation, and the two sample t-test was used to test the difference between treatment groups. Categorical variables were summarized by frequencies and percentages, and the proportions were calculated and compared between treatment groups using Fisher's Exact test.
Results
Trial treatment and control group
Application of PleuraSeal™ sealant
Variablea | PleuraSeal™ Group | Control Group | p-valueb |
|---|---|---|---|
20 | 20 | ||
Adjusted air leaks | |||
Total # of air leaks | 31 | 28 | NA |
Initial # of air leaks/patient | 1.6 (0.7) | 1.6 (0.5) | 1.00 |
# of air leaks/patient after additional treatment | 1.6 (0.6) | 1.4 (0.5) | 0.39 |
Initial grade of air leaks/patient | 1.3 (0.6) | 1.4 (0.5) | 0.77 |
Grade of air leaks/patient after additional treatment | 1.2 (0.4) | 1.1 (0.2) | 0.24 |
PleuraSeal™ application | 20 | - | NA |
Volume of sealant (ml)c | 2.8 (1.0) | - | - |
Duration (s)c | 43.0 (17.7) | - | - |
Second application (n) | 1 (5%) | - | - |
Micromyst applicator | 20 (100%) | - | - |
Air leak locations | NA | ||
Staple lines | 7 (23%) | - | - |
Suture lines | 2 (6%) | - | - |
Interfissure area | 16 (52%) | - | - |
Adhesiolysis | 2 (6%) | - | - |
Other manipulation | 4 (13%) | - | - |
Postoperative outcome
Post-operative complications and outcome
Variablea | Overall | PleuraSeal™ Group | Control Group | p-valueb |
|---|---|---|---|---|
40 | 20 | 20 | ||
Duration of chest tube (d) c , mean (SD) | ||||
Ventral chest tube removal (air leak) | 2.1 (1.2) | 3.9 (3.3) | 0.03 | |
Dorsal chest tube removal (drainage) | 5.1 (4.0) | 5.2 (3.4) | 0.94 | |
Chest tube on suction (h) c mean (SD) | 22.9 (1.8) | 49.7 (28.2) | <0.001 | |
Limiting factor for chest tube removal | ||||
Air leakage | 8 (20%) | 1 (5%) | 7 (35%) | 0.04 |
Drainage | 32 (80%) | 19 (95%) | 13 (65%) | 0.94 |
Total # of postoperative complications d | 21 (52.5%) | 10 (50%) | 11 (55%) | 1.000 |
Bronchogenic fistula | 1 (2.5%) | 0 (0%) | 1 (5%) | |
Chylothorax | 3 (7.5%) | 2 (10%) | 1 (5%) | |
Pneumonia | 6 (15%) | 4 (20%) | 2 (10%) | |
Mechanical Ventilation/ARDS | 1 (2.5%) | 0 (0%) | 1 (5%) | |
Tachyarrhythmia absoluta | 5 (12.5%) | 3 (15%) | 2 (10%) | |
Postoperative severe pain | 4 (10%) | 1 (5%) | 3 (15%) | |
Otherse | 1 (2.5%) | 0 (0%) | 1 (5%) | |
Length of stay(days) c | ||||
Potential hospitalization | 8.2 (4.6) | 10.3 (5.5) | 0.205 | |
Actual hospitalization | 9.9 (3.7) | 11.7 (4.4) | 0.178 | |
Intraoperative and postoperative air leakage and chest tube duration
Time of chest tube suction.
Duration of postoperative chest tube drainage for air leakages.
Conclusion
A simple, quick, and reproducible method to seal air leaks during thoracic procedures for lung resections could have a great impact on postoperative air leakage and clinical outcome. Ideally, such a product should bind rapidly to the tissue with strong adherence and not constrict lung volume expansion. It should be bacteriostatic and non-irritating, slowly and controllably break down in body fluids and be systemically non-toxic and inherently safe [9, 21]. The trial sealant PleuraSeal™ used in the study has been shown to have very good biocompatibility and has undergone extensive pre-clinical testing both in vitro [19], and in vivo [21] Furthermore, its components have been used in several medical applications [22, 23] and it has been proven to have strong adherence and flexibility in an ex-vivo porcine lung model, sealing coin size defects measuring approximately 5 mm in depth and 15 mm in diameter and with lung expansion up to 40 cm H2O. In this model, the trial sealant demonstrated 100% adherence and expansion without sealant tearing or delamination, and without restricting the normal expansion of the lung. The average burst strength pressure for the PleuraSeal™ sealant applied with the MicroMyst™ air-assisted applicator over the coin size defects was 291 cm H2O, 167 cm H2O, and 156 cm H2O at 0, 24, and 48 hours, respectively, which exceeds the maximal cough pressure at 2 days post-op of 102 cm H2O [24]. The mode of failure in all instances was cohesive rather than adhesive.
The study demonstrated a superior efficacy of the trial sealant as compared with standard surgical treatment for sustained sealing of postoperative air leakage following pulmonary resections. It enables an immediate and safe air tight seal intraoperatively with a high proportion of patients remaining air leak free, shorter air leak chest tube suction and duration, and may lead to a decrease in hospital length of stay. However, many factors can impact the length of stay for thoracic patients: postoperative air leaks, co-morbidities, the patient's social environment and healthcare reimbursement incentives. It should be noted that under the German Healthcare System there is currently no financial incentive to discharge patients from the hospital as soon as possible, as there is in the US system. One plausible explanation for the tendancy of shortened hospital length of stay in this study is that the earlier removal of the air leak chest tube and attached canister in the PSG increased patient mobility leading to a faster recovery. It is interesting that a shorter hospital length of stay of 1.8 days in the PSG corresponds exactly to the 1.8 days earlier removal of the air leak chest tube in the PSG.
However, the study has its limitations as far as the included study population is concerned. Since this is a preliminary study after introduction of this surgical device in patients undergoing lung resections only a small number of 20 patients as calculated above were included and compared to a case-match control cohort. Of note is that we could demonstrate statistical significance of p < 0.001 for our primary outcomes and showed reduced intraoperative air leakage and postoperative leak free through discharge.
The ease of use and effective sealing attributes of the PleuraSeal™ sealant enables a surgeon to change their mindset toward alveolar air leaks, and strive toward intra-operative leak free surgery. It is common practice to fill the chest cavity with warm saline to check intraoperatively for bronchopleural fistula and during this step one may also accurately identify the location of alveolar air leaks by gradually lowering the water level and identifying the source of air bubbles. The sealant may then be applied site specifically to cover all the identified air leaks and also prophylacticly to staple lines and manipulated tissue in less than one minute. Especially, since 52% of the intra-operative air leaks in the study were detected in the interfissure area which is difficult to access by standard suturing or fleece-bond sealants, PleuraSeal™ as a liquid sealant is ideal to seal air leaks in this interlobar space with its many anatomical variations. As part of this Protocol we resubmerged the lung parenchyma with warm saline and retested under pressure to confirm that all alveolar air leaks had been successfully halted with the study sealant, and in only one patient was an additional application of the trial sealant required to obtain intra-operative leak free surgery. We did not experience any pleural empyema, pneumothoraces, chest tube replacement, or re-operation in the presented study.
In summary, this liquid, hydrogel tissue sealant is safe and easy to use and has a significant impact on intra- and postoperative air leakage prevention as well as on early chest tube removal for air leaks. We also found a trend toward a reduced length of hospitalization, but this did not reach statistical significance.
Declarations
Acknowledgements
This study was funded by Covidien Surgical Devices. The authors are grateful to John Hauschild for his assistance in compiling the data for analysis and reviewing the manuscript.
Authors’ Affiliations
References
- Ciccone AM, Meyers BF, Guthrie TJ: Long-term outcome of bilateral lung volume reduction in 250 consecutive patients with emphysema. J Thorac Cardiovasc Surg. 2003, 125: 513-525. 10.1067/mtc.2003.147.View ArticlePubMedGoogle Scholar
- Okereke I, Murthy SC, Alster JM, Blackstone EH, Rice TW: Characterization and importance of air leak after lobectomy. Ann Thorac Surg. 2005, 79: 1167-1173. 10.1016/j.athoracsur.2004.08.069.View ArticlePubMedGoogle Scholar
- Serra-Mitjans M, Belda-Sanchis J, Rami-Porta R: Surgical sealant for preventing air leaks after pulmonary resections in patients with lung cancer. Cochrane Database Syst Rev. 2005, CD003051-Google Scholar
- Gomez-Caro A, Calvo MJ, Lanzas JT, Chau R, Cascales P, Parrilla P: The approach of fused fissures with fissureless technique decreases the incidence of persistent air leak after lobectomy. Eur J Cardiothorac Surg. 2007, 31: 203-208. 10.1016/j.ejcts.2006.11.030.View ArticlePubMedGoogle Scholar
- Passlick B, Born C, Haussinger K, Thetter O: Efficiency of video-assisted thoracic surgery for primary and secondary spontaneous pneumothorax. Ann Thorac Surg. 1998, 65: 324-327. 10.1016/S0003-4975(97)01128-4.View ArticlePubMedGoogle Scholar
- Abolhoda A, Liu D, Brooks A, Burt M: Prolonged air leak following radical upper lobectomy: an analysis of incidence and possible risk factors. Chest. 1998, 113: 1507-1510. 10.1378/chest.113.6.1507.View ArticlePubMedGoogle Scholar
- Hazelrigg SR, Nunchuck SK, LoCicero J: Video Assisted Thoracic Surgery Study Group data. Ann Thorac Surg. 1993, 56: 1039-1043. 10.1016/0003-4975(95)90011-X.View ArticlePubMedGoogle Scholar
- DeCamp MM, Blackstone EH, Naunheim KS: Patient and surgical factors influencing air leak after lung volume reduction surgery: lessons learned from the National Emphysema Treatment Trial. Ann Thorac Surg. 2006, 82: 197-206. 10.1016/j.athoracsur.2006.02.050.View ArticlePubMedGoogle Scholar
- Ranger WR, Halpin D, Sawhney AS, Lyman M, Locicero J: Pneumostasis of experimental air leaks with a new photopolymerized synthetic tissue sealant. Am Surg. 1997, 63: 788-795.PubMedGoogle Scholar
- Stephan F, Boucheseiche S, Hollande J: Pulmonary complications following lung resection: a comprehensive analysis of incidence and possible risk factors. Chest. 2000, 118: 1263-1270. 10.1378/chest.118.5.1263.View ArticlePubMedGoogle Scholar
- Ochroch A, Barnett R: Synthetic Sealants for Preventing Air Leaks after Pulmonary Resection. 2002, Official Newsletter of the Society of Cardiovascular AnesthesiologistsGoogle Scholar
- Brunelli A, Xiume F, Al RM, Salati M, Marasco R, Sabbatini A: Air leaks after lobectomy increase the risk of empyema but not of cardiopulmonary complications: a case-matched analysis. Chest. 2006, 130: 1150-1156. 10.1378/chest.130.4.1150.View ArticlePubMedGoogle Scholar
- Belboul A, Dernevik L, Aljassim O, Skrbic B, Radberg G, Roberts D: The effect of autologous fibrin sealant (Vivostat) on morbidity after pulmonary lobectomy: a prospective randomised, blinded study. Eur J Cardiothorac Surg. 2004, 26: 1187-1191. 10.1016/j.ejcts.2004.08.009.View ArticlePubMedGoogle Scholar
- Fabian T, Federico JA, Ponn RB: Fibrin glue in pulmonary resection: a prospective, randomized, blinded study. Ann Thorac Surg. 2003, 75: 1587-1592. 10.1016/S0003-4975(02)04994-9.View ArticlePubMedGoogle Scholar
- Anegg U, Lindenmann J, Matzi V, Smolle J, Maier A, Smolle-Juttner F: Efficiency of fleece-bound sealing (TachoSil) of air leaks in lung surgery: a prospective randomised trial. Eur J Cardiothorac Surg. 2007, 31: 198-202. 10.1016/j.ejcts.2006.11.033.View ArticlePubMedGoogle Scholar
- Lang G, Csekeo A, Stamatis G: Efficacy and safety of topical application of human fibrinogen/thrombin-coated collagen patch (TachoComb) for treatment of air leakage after standard lobectomy. Eur J Cardiothorac Surg. 2004, 25: 160-166. 10.1016/j.ejcts.2003.11.018.View ArticlePubMedGoogle Scholar
- Porte HL, Jany T, Akkad R: Randomized controlled trial of a synthetic sealant for preventing alveolar air leaks after lobectomy. Ann Thorac Surg. 2001, 71: 1618-1622. 10.1016/S0003-4975(01)02468-7.View ArticlePubMedGoogle Scholar
- Wain JC, Kaiser LR, Johnstone DW: Trial of a novel synthetic sealant in preventing air leaks after lung resection. Ann Thorac Surg. 2001, 71: 1623-1628. 10.1016/S0003-4975(01)02537-1.View ArticlePubMedGoogle Scholar
- Campbell P: Evaluation of Absorbable Surgical Sealants: In-vitro Testing. In-vitro testing. Edited by: Bennet S, Sawhney A. 2007, Covidien Laboratories, Mansfield, MAGoogle Scholar
- Macchiarini P, Wain J, Almy S, Dartevelle P: Experimental and clinical evaluation of a new synthetic, absorbable sealant to reduce air leaks in thoracic operations. J Thorac Cardiovasc Surg. 1999, 117: 751-758. 10.1016/S0022-5223(99)70296-5.View ArticlePubMedGoogle Scholar
- Locicero J: Evaluation of PleuraSeal™ Sealant System as a Thoracic Sealant in a Canine Lung Resection Model. North American Science Associates (NAMSA). Edited by: Campbell P, Muench TR. 2007, Northwood, OhioGoogle Scholar
- Bennett SL, Melanson DA, Torchiana DF, Wiseman DM, Sawhney AS: Next-generation hydrogel films as tissue sealants and adhesion barriers. J Card Surg. 2003, 18: 494-499. 10.1046/j.0886-0440.2003.00303.x.View ArticlePubMedGoogle Scholar
- Preul MC, Bichard WD, Spetzler RF: Toward optimal tissue sealants for neurosurgery: use of a novel hydrogel sealant in a canine durotomy repair model. Neurosurgery. 2003, 53: 1189-1198. 10.1227/01.NEU.0000089481.87226.F7.View ArticlePubMedGoogle Scholar
- Byrd RB, Burns JR: Cough dynamics in the post-thoracotomy state. Chest. 1975, 67: 654-657. 10.1378/chest.67.6.654.View ArticlePubMedGoogle Scholar
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This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
